Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin
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منابع مشابه
Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin.
Poly(A)-rich RNA isolated from ripening ovaries of snowdrop (Galanthus nivalis L.) yielded a single 17-kDa lectin polypeptide upon translation in a wheat-germ cell-free system. This lectin was purified by affinity chromatography. Translation of the same RNA in Xenopus leavis oocytes revealed a lectin polypeptide which was about 2 kDa smaller than the in vitro synthesized precursor, suggesting t...
متن کاملBiosynthesis of the Snowdrop (Galanthus nivalis) Lectin in Ripening Ovaries.
The biosynthesis and processing of the Galanthus nivalis agglutinin were studied in vivo in ripening snowdrop ovaries. Using labeling and pulse chase labeling experiments it could be demonstrated that the snowdrop lectin is synthesized as a precursor of relative molecular weight (M(r)) 15,000 which is posttranslationally converted into the authentic lectin polypeptide of M(r) 13,000 with a half...
متن کاملA 90-day safety study in Wistar rats fed genetically modified rice expressing snowdrop lectin Galanthus nivalis (GNA).
Genetically modified plants expressing insecticidal traits offer a new strategy for crop protection, but at the same time present a challenge in terms of food safety assessment. The present 90-day feeding study was designed to assess the safety of a rice variety expressing the snowdrop Galanthus nivalis lectin (GNA lectin), and forms part of a EU-funded project where the objective has been to d...
متن کاملEffects of Snowdrop Lectin (Galanthus nivalis Agglutinin) Expressed in Transgenic Sugarcane on Fitness of Cotesia flavipes (Hymenoptera: Braconidae), a Parasitoid of the Nontarget Pest Diatraea saccharalis (Lepidoptera: Crambidae)
Cotesia flavipes (Cameron) is a parasitoid responsible for maintaining populations of sugarcane borer, Diatraea saccharalis (F.), below economic levels in south Texas sugarcane Þelds. Transgenic sugarcane expressing the snowdrop lectin (Galanthus nivalis agglutinin, GNA) was developed against the Mexican rice borer, Eoreuma loftini (Dyar), the primary pest of south Texas sugarcane. The potentia...
متن کاملEffect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine.
Diets containing genetically modified (GM) potatoes expressing the lectin Galanthus nivalis agglutinin (GNA) had variable effects on different parts of the rat gastrointestinal tract. Some effects, such as the proliferation of the gastric mucosa, were mainly due to the expression of the GNA transgene. However, other parts of the construct or the genetic transformation (or both) could also have ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1991
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1991.tb16339.x